Vladimir Shafirovich

Associate Research Professor

Education

M.S., Moscow State University

Ph.D., Institute of Chemical Physics, Chernogolovka

D. Sc., Institute of Chemical Physics, Chernogolovka

Areas of Research/Interest

DNA damage is known to be induced by reactive intermediates (free radicals) that are overproduced in humans at sites of inflammation. The major focus is to understand the mechanisms of formation of the oxidatively generated DNA lesions and their repair in free DNA and in DNA wrapped around histone octamers as in cellular chromatin.

Selected Publications

Kolbanovskiy M, Aharonoff A, Sales AH, Geacintov NE, Shafirovich V. (2020) Remarkable Enhancement of Nucleotide Excision Repair of a Bulky Guanine Lesion in a Covalently Closed Circular DNA Plasmid Relative to the Same Linearized Plasmid. Biochemistry, 59, 2842-2848. PMCID: PMC7444659.

Kolbanovskiy M, Shim Y, Min JH, Geacintov NE, Shafirovich V. (2020) Inhibition of Excision of Oxidatively Generated Hydantoin DNA Lesions by NEIL1 by the Competitive Binding of the Nucleotide Excision Repair Factor XPC-RAD23B. Biochemistry, 59, 1728-1736. PMCID: PMC7413705.

Chatgilialoglu C, Ferreri C, Geacintov NE, Krokidis MG, Liu Y, Masi A, Shafirovich V. Terzidis MA, Tsegay PS. (2019) 5',8-Cyclopurine Lesions in DNA Damage: Chemical, Analytical, Biological, and Diagnostic Significance. Cells, 8, 513. PMCID: PMC6628319.

Shafirovich V, Kropachev K, Kolbanovskiy M, Geacintov NE. (2019) Excision of Oxidatively Generated Guanine Lesions by Competing Base and Nucleotide Excision Repair Mechanisms in Human Cells. Chem Res Toxicol. 32, 753-761. 

Shafirovich V, Kolbanovskiy M, Kropachev K, Liu Z, Cai Y, Terzidis MA, Masi A, Chatgilialoglu C, Amin S, Dadali A, Broyde S, Geacintov NE. (2019) Nucleotide Excision Repair and Impact of Site-Specific 5',8-Cyclopurine and Bulky DNA Lesions on the Physical Properties of Nucleosomes. Biochemistry, 58, 561-574.  PMCID: PMC6373774.

Kolbanovskiy M, Chowdhury MA, Nadkarni A, Broyde S, Geacintov NE, Scicchitano DA, Shafirovich V. (2017) The nonbulky DNA lesions spiroiminodihydantoin and 5-guanidinohydantoin significantly block human RNA polymerase II elongation in vitro. Biochemistry, 56, 3008-3018.

Shafirovich V, Geacintov NE. (2016) Removal of oxidatively generated DNA damage by overlapping repair pathways. Free Radic Biol Med. 107, 53-61. PMCID: PMC5418118.

Lee YA, Lee YC, Geacintov NE, Shafirovich V. (2016) Translesion synthesis past guanine(C8)-thymine(N3) intrastrand cross-links catalyzed by selected A- and Y-family polymerases. Mol Biosyst. 12, 1892-1900. PMCID: PMC4879090.

Shafirovich V, Kropachev K, Anderson T, Liu Z, Kolbanovskiy M, Martin BD, Sugden K, Shim Y, Chen X, Min JH, Geacintov NE. (2016). Base and nucleotide excision repair of oxidatively generated guanine Lesions in DNA. J Biol Chem. 291, 5309-5319. PMCID: PMC4777862.

Talhaoui, I., Shafirovich, V., Liu, Z., Saint-Pierre, C., Akishev, Z., Matkarimov, B. T., Gasparutto, D., Geacintov, N. E., and Saparbaev, M. (2015) Oxidatively generated guanine(C8)-thymine(N3) intrastrand cross-links in double-stranded DNA are repaired by base excision repair pathways. J Biol Chem. 14610-14617. 

Rokhlenko, Y., Cadet, J., Geacintov, N.E., and Shafirovich, V. (2014). Mechanistic aspects of hydration of Guanine radical cations in DNA. J Am Chem Soc 136, 5956-5962. PMC4004273.

Cadet, J., Wagner, J.R., Shafirovich, V., and Geacintov, N.E. (2014). One-electron oxidation reactions of purine and pyrimidine bases in cellular DNA. Int J Radiat Biol. 90, 423-432. PMC4047668.

Kropachev, K., Ding, S., Terzidis, M.A., Masi, A., Liu, Z., Cai, Y., Kolbanovskiy, M., Chatgilialoglu, C., Broyde, S., Geacintov, N.E., Shafirovich, V. (2014). Structural basis for the recognition of diastereomeric 5',8-cyclo-2'-deoxypurine lesions by the human nucleotide excision repair system. Nucleic Acids Res 42, 5020-5032. PMC4041128.

Uvaydov, Y., Geacintov, N.E., and Shafirovich, V. (2014). Generation of guanine-amino acid cross-links by a free radical combination mechanism. Phys Chem Chem Phys. 16, 11729-11736. PMC4068030.

Khutsishvili, I., Zhang, N., Marky, L.A., Crean, C., Patel, D.J., Geacintov, N.E., and Shafirovich, V. (2013). Thermodynamic profiles and nuclear magnetic resonance studies of oligonucleotide duplexes containing single diastereomeric spiroiminodihydantoin lesions. Biochemistry 52, 1354-1363. PMC3588586.

Madugundu, G.S., Wagner, J.R., Cadet, J., Kropachev, K., Yun, B.H., Geacintov, N.E., and Shafirovich, V. (2013). Generation of guanine-thymine cross-links in human cells by one-electron oxidation mechanisms. Chem Res Toxicol 26, 1031-1033. PMC3812307

Rokhlenko, Y., Geacintov, N.E., and Shafirovich, V. (2012). Lifetimes and reaction pathways of guanine radical cations and neutral guanine radicals in an oligonucleotide in aqueous solutions. J Am Chem Soc 134, 4955-4962. PMC3303975.

Liu, Y., Liu, Z., Geacintov, N.E., and Shafirovich, V. (2012). Proton-coupled hole hopping in nucleosomal and free DNA initiated by site-specific hole injection. Phys Chem Chem Phys 14, 7400-7410. PMC3674890.

Ding, S., Kropachev, K., Cai, Y., Kolbanovskiy, M., Durandina, S.A., Liu, Z., Shafirovich, V., Broyde, S., and Geacintov, N.E. (2012). Structural, energetic and dynamic properties of guanine(C8)-thymine(N3) cross-links in DNA provide insights on susceptibility to nucleotide excision repair. Nucleic Acids Res 40, 2506-2517. PMC3315297.

Lee, Y.A., Liu, Z., Dedon, P.C., Geacintov, N.E., and Shafirovich, V. (2011). Solvent exposure associated with single abasic sites alters the base sequence dependence of oxidation of guanine in DNA in GG sequence contexts. ChemBioChem 12, 1731-1739. PMC3517150.

Yun, B.H., Geacintov, N.E., and Shafirovich, V. (2011). Generation of guanine-thymidine cross-links in DNA by peroxynitrite/carbon dioxide. Chem Res Toxicol 24, 1144-1152. PMC3138886.

Shao, J., Geacintov, N.E., and Shafirovich, V. (2010). Oxidation of 8-oxo-7,8-dihydro-2'-deoxyguanosine by oxyl radicals produced by photolysis of azo compounds. Chem Res Toxicol 23, 933-938. PMC2873103.

Shao, J., Geacintov, N.E., and Shafirovich, V. (2010). Oxidative modification of guanine bases initiate by oxyl radicals derived from photolysis of azo compounds. J Phys Chem B 114, 6685-6692. PMC2879049.