Nathaniel J. Traaseth

Professor of Chemistry

Education

B.S., University of Minnesota-Duluth

Ph.D., University of Minnesota

Postdoctoral Fellow, University of Minnesota

Areas of Research/Interest

NMR Spectroscopy, Physical Chemistry, Chemical Biology, Biophysics, Structural Biology

External Affiliations

Faculty Member of the Sackler Institute of Graduate Biomedical Sciences

Fellowships/Honors

NIH K22 Career Development Award from NIAID (2011-2012)

Paul D. Boyer/James B. Peter Award (2008)

Overend Award (2007)

AHA Pre-Doctoral Fellowship (2005-2007)

NIH Neuro-Physical-Computational Sciences Grant (2004-2005)

Izaak Koltoff Fellowship (2003-2004)

American Institute of Chemist's Award (2003)

 

Publications

Chen L+, Marsiglia WM+, Chen H+, Katigbak J, Erdjument-Bromage H, Kemble DJ, Fu L, Ma J, Sun G, Zhang Y, Liang G, Neubert TA, Li X, Traaseth NJ*, Mohammadi M* (2020). Molecular basis for receptor tyrosine kinase A-loop tyrosine transphosphorylation. Nat Chem Biol, in-press. (+equal contribution).

Marsiglia WM, Qamra R, Jackson KM, Traaseth NJ (2020). A CURE biochemistry laboratory module to study protein-protein interactions by NMR spectroscopy. J Chem Educ, in-press.

Leninger M+, Sae Her A+, Traaseth NJ (2019). Inducing conformational preference of the membrane protein transporter EmrE through conservative mutations. eLife, 8:e48909. (+equal contribution)

Marsiglia WM, Katigbak J, Zheng S, Mohammadi M, Zhang Y, Traaseth NJ (2019). A Conserved Allosteric Pathway in Tyrosine Kinase Regulation. Structure, 27(8):1308-1315.

Yang W, Lee JS, Leninger M, Windschuh J, Traaseth NJ, Jerschow A (2019). Magnetization Transfer in Liposome and Proteoliposome Samples that Mimic the Protein and Lipid Composition of Myelin. NMR in Biomedicine, 32, e4097.

Leninger M+, Marsiglia WM+, Jerschow A, Traaseth NJ. Multiple Frequency Saturation Pulse Reduce CEST Acquisition Time for Quantifying Conformational Exchange in Biomolecules. JBiomolec NMR, (2018), 71, 19-30. (+equal contribution)

Gao A, Vasiley N, Luciano DJ, Levenson-Palmer R, Richards J, Marsiglia WM, Traaseth NJ, Balasco JG, Serganov A. Structural and Kinetic Insights into Stimulation of RppH-Dependent RNA Degradation by the Metabolic Enzyme DapF. Nucleic Acids Res, (2018), 46, 6841-6856.

Banigan JR, Leninger M, Sae Her A, Traaseth NJ. Assessing Interactions Between Polytopic Membrane Proteins and Lipid Bilayers using Differential Scanning Calorimetry and Solid-State NMR. J Phys Chem B, (2018), 122, 2314-2322.

Leninger L, Traaseth NJ. NMR spectroscopy approach to study the structure, orientation, and mechanism of the multidrug exporter EmrE. Methods Mol Biol, 2018, 1700, 83-96. Abramov G, Traaseth NJ. Afterglow Solid-State NMR Spectroscopy. Methods Mol Biol, 2018, 1686, 55-66.

Chen H+, Marsiglia WM+, Cho M-K, Huang Z, Deng J, Blais S, Gai W, Bhattacharya S, Neubert TA, Traaseth NJ*, Mohammadi M* (2017) Elucidation of a Four-Switch Allosteric Network in FGF Receptor Tyrosine Kinases. eLife, 6, e21137. doi: 10.7554/eLife.21137. (+equal contribution)

Gayen A+, Leninger L+, Traaseth NJ* (2016) Protonation of a Glutamate Residue Modulates the Dynamics of the Drug Transporter EmrE. Nat Chem Biol, 12, 141-5. *Cover Article (+equal contribution)

Craven TW, Cho M-K, Traaseth NJ, Bonneau R*, Kirshenbaum K* (2016) A miniature Protein Stabilized by a Cation-π Interaction Network Core. J Am Chem Soc, 138, 1543-50.

Huang Z, Marsiglia WM, BasuRoy U, Rahimi N, Ilghari D, Wang H, Chen H, Gai W, Blais S, Neubert TA, Mansukhani A, Traaseth NJ, Li X*, Mohammadi M* (2016). Two FGF Receptor kinases act in concert to recruit and transphosphorylate Phospholipase Cγ. Mol Cell, 61, 98-110.

Banigan JR, Gayen A, Cho MK, Traaseth NJ (2015) A Structured Loop Modulates Coupling Between the Substrate-Binding and Dimerization Domains in the Multidrug Resistance Transporter EmrE. J Biol Chem, 290, 805-14.

Traaseth NJ, Banigan JR, Leninger M (2015) Exploring Transporters within the Small Multidrug Resistance Family Using Solid-State NMR Spectroscopy. eMagRes, 4, 551-9.

Banigan JR, Gayen A, Traaseth NJ (2015).  Correlating Lipid Bilayer Fluidity with Sensitivity and Resolution of Polytopic Membrane Protein Spectra by Solid-State NMR Spectroscopy. Biochim Biophys Acta, 1848, 334-41.

Cho MK, Gayen A, Banigan JR, Leninger M, Traaseth NJ (2014). The Intrinsic Conformational Plasticity of Native EmrE Provides a Pathway for Multidrug Resistance. J Am Chem Soc, 136, 8072-80.

Kushala S, Lao BB, Henchey LK, Dubeya R, Mesallati H, Traaseth NJ, Olenyuka BZ, Arora PS (2013). Protein Domain Mimetics as In Vivo Modulators of Hypoxia-Inducible Factor Signaling. Proc Natl Acad Sci, 110, 15602-7.

Gayen A, Banigan JR, Traaseth NJ (2013). Ligand-Induced Conformational Changes of the Multidrug Resistance Transporter EmrE Probed by Oriented Solid-State NMR Spectroscopy. Angew Chemie Int Ed, 52, 10321-4.

Chen H, Huang Z, Dutta K, Blais S, Neubert TA, Li X, Cowburn D, Traaseth NJ, Mohammadi M (2013) Cracking the Molecular Origin of Intrinsic Tyrosine Kinase Activity through Pathogenic Gain-of-Function Mutations. Cell Reports, 4(2), 376-84.

Banigan JR, Gayen A, Traaseth NJ (2013). Combination of 15N reverse labeling and afterglow spectroscopy for assigning membrane protein spectra by magic-angle-spinning solid-state NMR: application to the multidrug resistance protein EmrE. J Biomolec NMR, 55, 391-9.

Banigan JR, Traaseth NJ (2012). Utilizing Afterglow Magnetization from Cross-Polarization Magic-Angle-Spinning Solid-State NMR Spectroscopy to Obtain Simultaneous Heteronuclear Multidimensional Spectra. J Phys Chem B, 116, 7138-44.