My research interests focus on gap junctions which are involved in cell-to-cell communication. Regulating the chemical and physical properties of gap junctions are the different connexin proteins. Unique communication compartments can be formed when gap junctions are assembled from various connexins.
Presently, I am examining the implications of gap junctions on cell differentiation and development using the testis as a model. Organized in the seminiferous tubule and supported by Sertoli cells are some 63 different germ cell types. The germ cells are arranged and organized in the seminiferous epithelium for an ordered development and differentiation into spermatozoa. We are currently determining gap junction’s role in the formation of specific communication compartments and how gap junctions regulate and support specific spermatogenic cells. Gap junction assembly, connexin composition, and the chemical and physical properties of homotypic - heterotypic gap junctions will be examined biochemically and ultrastructurally.
Electron Microscope and Image Analysis Facility:
The facility has a confocal microscope and three electron microscopes: two TEM's and a SEM. Support equipment include ultramicrotomes, paraffin micotome, high vacuum evaporator, sputter coater, critical point dryer, and a low temperature UV chamber for immuno EM. The two modern EM’s are capable of high resolution imaging and capturing digital images. The SEM supports elemental image mapping/analysis and imaging of frozen samples.
Transmission Electron Microscopes:
Phillips CM-10. Scanning Electron Microscopes: AMRAY 1850-FE with secondary, Robinson backscatter, and EDX detectors and cryo-chamber
Confocal Microscope System:
Leica TCS SP2 confocal system for available inverted and upright microscopes.
Image analysis workstation.
Confocal Calendar website: labgear.bio.nyu.edu