THIS SEMINAR HAS BEEN RESCHEDULED FROM OCTOBER 2, 2020.
Jeffrey W. Bode of ETH, Zurich, will deliver a virtual seminar entitled, "Tailor Made Proteins by Synthesis and Conjugation." Hosted by Bobby Arora.
For more information about Jeff Bode, click here.
Zoom Link: https://nyu.zoom.us/j/97010026579?pwd=SC9oaTR0ekV3YlJQZ252SGtsek1rZz09
Abstract: The chemical synthesis of proteins is a powerful tool for elucidating biological processes and has great potential for the preparation of the next generation of therapeutic proteins. Solid phase peptide synthesis (SPPS) can routinely provide peptide segments of about 40 amino acid residues but is not suited for the preparation of proteins. Our group has developed a chemoselective amide-forming reaction of a-ketoacids and hydroxylamines (KAHA ligation), enabling the synthesis of proteins up to ~200 residues by combining unprotected peptide segments – without coupling reagents or side chain protecting groups. By establishing robust, scalable routes to the key linkers and building blocks, we can now routinely synthesize small proteins using Fmoc-SPPS – without the need for any non-standard workflows or post synthesis manipulations.
As part of a larger program aimed at the construction of protein–protein conjugates, we have also devised a new approach to the site specific attachment of peptides and proteins to recombinant proteins that bear a short (4 residue) recognition sequence. This approach – Lysine Acylation with Conjugating Enzymes (LACE) – offers the ability to directly modify a single lysine residue in folded proteins and is suitable for both labelling and monoubiquitination of protein targets.