The NYU Chemistry Department Shared Instrumentation Facility is located in the Silver Center. The facility houses NMR spectrometers, mass spectrometers, X-ray diffractometers, calorimeters, electronic and vibrational spectrophotometers. The instrumentation facility is managed by two Ph.D. staff scientists and three support staff members. These full-time staff members are dedicated toward student training and maintaining instrumentation within the facility.
NYU is a member of the New York Structural Biology Center and PI laboratories have access to the high field NMR spectrometers and cryoelectron microscopes located within this facility. The Center is managed by several Ph.D. NMR staff scientists with additional supporting staff members.
The GenCore Facility supports the research projects of NYU Biology researchers and collaborators. All sequencing services and shared equipment are run, monitored and maintained by a highly skilled technical staff (Manager and two Laboratory Technicians) overseen by the Faculty Director of Sequencing and Faculty Director of Share Equipment. The staff has extensive expertise in experimental design, NGS library construction, operation of NGS sequencing equipment, equipment training and running of all shared equipment.
Flow cytometry facility: This facility is located in the Skirball Institute. It contains a MoFlo high speed sorter, an Epics Elite cell sorter equipped with three lasers, a BD LSR cytometer, a FACScan flow cytometer and a FACSCalibur. Proteomics Facility: This facility provides: MALDI-TOF mass spectrometry, Q-TOF mass spectrometry (MS/MS peptide sequencing, proteolysis and identification of proteins from SDS-PAGE gels, electrospray mass spectrometry (single quadrupole) and analytical HPLC.
Analytical Chemistry Core NYU School of Medicine’s Analytical Chemistry Core provides metals and elemental analysis of environmentally, experimentally, or pharmacologically exposed cells and tissues. We also analyze other biological and environmental samples. Our metals analysis laboratory features advanced X-ray fluorescence (XRF) spectrometry and inductively coupled plasma mass spectrometry (ICP-MS).
Anti-Infectives Screening Core NYU School of Medicine’s Anti-infectives Screening Core facilitates preclinical drug development for neglected parasitic diseases.
We enable quick and accurate in vitro and in vivo testing of drugs and other candidates, such as antibodies and peptides, against diseases that have murine models of infection. As a researcher, you have access to the specialized facilities and expertise available in NYU School of Medicine’s Department of Microbiology.
Applied Bioinformatics Laboratories NYU Langone’s Applied Bioinformatics Laboratories provide computational analysis for high-throughput genomic data, including but not limited to, next-generation sequencing data. Our mission is to accelerate scientific discoveries by guiding experimental design, performing robust data quality assessment, and carrying out comprehensive computational analyses.
Center for Biospecimen Research & Development NYU School of Medicine’s Center for Biospecimen Research and Development was launched as a partnership between the Office of Science and Research and the Department of Pathology. Our center’s mission is to drive scientific discovery through the use of human specimens. We help our investigators facilitate translational research, match human specimen resources to scientific needs, foster multi-investigator collaborative projects, and catalyze scientific innovation. Our center is also focused on increasing clinical trial support for investigator-initiated trials, improving researcher access to existing biospecimen resources, and enhancing institution-wide tissue-banking efforts and capacity.
Cytometry & Cell Sorting Laboratory Researchers partnering with NYU Langone’s Cytometry and Cell Sorting Laboratory have access to the most powerful and adaptable flow cytometry and cell sorting technologies and instruments available. If your research requires cytometric analysis, our state-of-the-art instruments acquire optical measurements using different lasers to detect fluorophores with a high level of precision.
DataCore is a resource launched by NYU Langone, based in the Information Technology department in close collaboration with the Clinical and Translational Science Institute and NYU School of Medicine’s Department of Population Health. DataCore integrates expertise and resources from multiple departments, divisions, and centers at NYU Langone. Our comprehensive services include providing electronic case report forms, database design, and data management and analysis. Studies may range from small, single-site trials to large, multicenter randomized clinical trials.
Experimental Pathology Research Laboratory NYU School of Medicine’s Experimental Pathology Research Laboratory provides researchers with a range of services, access to advanced instruments, and training to facilitate the production of high-quality animal and human tissue samples for morphological and molecular analyses.
Genome Technology Center NYU School of Medicine’s Genome Technology Center provides faculty, staff, and partnering researchers with a range of services related to genome, epigenome, and transcriptome analysis. We offer numerous advanced technologies, including the following:
- Illumina deep sequencing and sample preparation for a variety of applications, including DNA and RNA sequencing (DNA- and RNA-seq), exome sequencing, targeted capture, chromatin immunoprecipitation sequencing (ChIP-seq), methylation sequencing (Methyl-seq), metagenomics, and many others
- Automation of Illumina library and targeted capture preps, including 16S ribosomal RNA (rRNA) sequencing
- Oxford Nanopore sequencing (long reads)
- Bio-Rad Droplet Digital™ polymerase chain reaction (PCR)
- Nanostring nCounter
- Single-cell RNA- and DNA-seq using the C1™ Auto Prep System from Fluidigm, and 10x Genomics Chromium™ System
Genotyping Core Laboratory provides fast, accurate, convenient, and affordable genotyping technologies. We use conventional polymerase chain reaction (PCR) and gel electrophoresis methods. By pooling samples from a group of users, we can better ensure accuracy, cost-effectiveness, and quality control. Our team of professional technicians and scientists has years of genotyping and troubleshooting experience, averaging 1,500 tails per month from an expanding library of more than 175 protocols.
High Performance Computing Core is NYU Langone’s central resource for performing computational research at scale and for analyzing big data. We provide a range of integrative services using supercomputing to perform basic, translational, and clinical informatics research. As a core facility, we are well-positioned to advise scientists at NYU Langone on best practices for improving the quality and range of their research. NYU School of Medicine currently operates a terascale HPC cluster, called Phoenix, and is in the process of transitioning from Dell EMC Isilon to IBM Elastic Storage Server (ESS) storage technology. In 2018 we will put into production a state-of-the-art 90-node Cray HPC system including a 4 PB ESS-based IBM Spectrum Scale parallel file system complemented by a 500 TB ESS-based IBM Spectrum Scale solid–state drive scratch space. This new petascale supercomputer will deliver expanded computational capabilities, in particular in its graphics processing unit–based subsystems. The new HPC cluster will be the backbone of our big data strategy; additional growth is planned in two expansion phases, in 2019 and 2021.
High Throughput Biology Laboratory supports researchers by providing high throughput and high content screening services and equipment. These include the latest in automated liquid handling, imaging technologies, and multimode plate readers. In addition, we deliver logistical, technical, and bioinformatics support in the performance and interpretation of your experiments. Our services include the following:
- whole genome and custom small interfering RNA (siRNA) and microRNA (miRNA) human and mouse libraries
- automated liquid dispensing starting at 0.1 µl and microplate washing
- robotic plate replication, reformatting, and media transfers
- multimode plate readers (luminescence, fluorescence, absorbance, AlphaScreen®, infrared)
- assay development, screening, data analysis, and visualization support
- automated tissue and cellular imaging
- digital image analysis (such as viability, colony count, colocalization, and neurite outgrowth)
- plate preparation for enzyme-linked immunosorbent assay (ELISA) and screening of small molecules, biologics, and other assays
- training for independent equipment usage or supported and full service assay development and screening
Metabolomics Laboratory offers custom metabolomics services to our investigators and the greater scientific community. We specialize in mass spectrometry–based metabolomics, offering global (untargeted) and targeted methods for metabolite identification and quantification. Using a custom-targeted method, we can perform absolute or relative quantification, flux analysis, and biotransformation analysis. Our untargeted platform enables the detection of several thousand metabolite peaks from a typical sample of cells or tissue, focusing on either hydrophilic or hydrophobic metabolites. These data can be used to identify differentially regulated features, candidate markers, metabolite structures, and more.
Microscopy Laboratory is a shared resource offering comprehensive light and electron microscopy technologies. We partner with faculty, staff, and researchers at NYU Langone, as well as investigators from other institutions in the metropolitan area. Our scientists use powerful light microscopes and electron microscopes at resolutions ranging from centimeters to angstroms, providing clear and detailed images. Transmission and scanning electron microscopes, Zeiss LSM-510 and 710 confocal microscopes with fluorescence correlation spectroscopy modules, Olympus/custom two-photon microscope with cooled CCD and intensified CCD cameras, BioRad two photon microspcope system with 4 external PMTs, Rigaku RU-200 rotating anode with Rigaku Osmic Confocal Blue optics, Rigaku R-AXIS IIC image plate detector, Oxford Cryostream cooling system. Trainees also have access to the full capabilities of the New York Structural Biology Center, of which NYU is a founding partner.
Precision Immunology Laboratory is a shared resource that studies immune cell phenotype and function using state-of-the-art technology. Our mission is to comprehensively characterize antitumor immune responses, determine mechanisms of immune escape and evasion, and develop biomarkers that predict patient outcomes or possible toxicities. We offer a wide range of services for immune monitoring.
Preclinical Imaging Laboratory provides investigators with access to state-of-the-art imaging technologies and strategies to image live animals on the organ, tissue, cell, or molecular level. Our technologies are indispensable to researchers studying and monitoring disease in small living subjects. The lab is equipped with powerful instruments for micromagnetic resonance imaging, micropositron emission tomography, X-ray microcomputed tomography, bioluminescence and fluorescence scanning, and high-frequency ultrasound.
Proteomics Laboratory offers investigators specialized expertise for the analysis of proteins and peptides using mass spectrometry. Our laboratory continually develops new methods and customized approaches for proteomic analysis and suggests experimental strategies and sample preparation prior to mass spectrometry analysis. We offer many different technologies, including the following:
- comprehensive protein identification
- analysis of affinity purified complexes
- characterizing protein post-translational modifications
- de novo sequencing
- label and label-free quantitation
- multiplexed quantitation
- global phosphorylation and ubiquitin analysis
- analysis of laser-capture microdissected formalin-fixed paraffin-embedded tissue
- secretome analysis
- crosslinking analysis
- disulfide mapping
Rodent Genetic Engineering Laboratory is a resource for researchers who want to apply advanced molecular genetic techniques in rodent models of physiology and disease. Our lab is experienced at generating novel mutant mouse strains by using gene targeting in embryonic stem cells (ESCs) and by developing novel methods of modifying rodent genomes and assaying genetically modified cells in vivo. Available advanced technologies include CRISPR/Cas9, a proprietary rapid strain-creation method known as the EZ-Mouse Model, tetraploid complementation systems, GEMMs-ESC Speedy Models, regulated gene expression by high-throughput RNA interference assay in mice, safe-harbor knock-in gene editing using the Rosa26 and other loci, recombinase-mediated cassette exchange, Inducible Tet-On® and Tet-Off® Gene Expression Systems, homologous recombination, induced pluripotent stem cells, assisted reproductive technologies, and vitrification systems.
Small Instrument Fleet Available instruments include the following:
- Applied Biosystems StepOne™ Real-Time PCR System
- Bio-Rad CFX96 Touch™ Real-Time PCR Detection System
- Diagenode Biorupter®
- EVOS® FL Color Imaging System
- GE Typhoon Trio™
- LI-COR Odyssey® Fc Imaging System
- LI-COR Odyssey® Infrared Imaging System
- FlexStation® 3 Multi-Mode Microplate Reader
- GenePix® 4000B Microarray Scanner
- NanoDrop™ 8000 UV-Vis Spectrophotometer
- Agilent 2100 Bioanalyze
Protein Analysis Facility assists scientists at NYU with their research by helping them to characterize proteins of interest. Currently we can provide the following on a fee-per-service basis:
- MALDI-TOF mass spectrometry
- Proteolysis and identification of proteins from SDS-PAGE gels
- Phosphoprotein analysis
The NYU-HHC Clinical and Translational Science Institute (CTSI) is a major new resource available to the proposed training program. This Institute has developed an expanded infrastructure and additional resources to support novel translational and clinical science and education of investigators. The resources include support for 1) the Center for Health Informatics and Bioinformatics (CHIBI) which provides new approaches to designing experiments, gathering and analyzing data and increasing the scope of clinical and translational research; 2) expanded biostatistical services and training; 3) the Clinical Research Center, staffed with experienced research nurses in both inpatient and outpatient settings; and 4) the Translational Research Education Core, which provides resources for promoting the education of graduate students, postdoctoral fellows and clinical investigators in the techniques and approaches to translational research as well as providing training for mentors and mentees designed to maximize educational and training opportunities.
Genomics Core Facility: contains the Affymetrix GenChip Bioinformatics system 1, as well as a BioRad Arrayer and a Genomic Solution LSIV Microanalyzer and the associate software, an Axon 400B scanner with GenePix Pro software and a Pyrosequencer. The facility provides Next-Gen DNA sequence analysis through Illumina and Roche 454 genome analyzers. The facility also provides access to real time PCR and a Beckman robotic system.
RNA Interference Core Facility: This facility performs automated genome-wide RNA interference screens using human, mouse, Drosophila, and C. elegans oligonucleotide libraries and supports automated luciferase, fluorescent, and microscope-based readouts.
Library Facilities: The School of Medicine Library is located on three floors of the Medical Science Building offering immediate access to more than 156,000 volumes and 2,000 current titles. Photoduplication and a computer-media center with a separate computer classroom and computer reference workstations are also located in the library to accommodate trainees’ study and research needs. The library also provides internet access to nearly all relevant scientific journals to trainees both on and off campus.
Animal Imaging: Several IVIS imaging systems with living image software for in vivo detection of bioluminescence reporters including renilla and firefly luciferase and GFP plus X-ray analysis of small animals; VisualSonics Vevo 660 ultrasound imager with Doppler and image guided injection capability for imaging of rodents; and a SMIS 300Mhz MRI imaging console with a 7T horizontal bore magnet for MRI imaging of rodents and small animals.
Tissue Acquisition and Banking Service: This resource includes human tumor banks supported by the NYU Cancer Institute permits the retrieval from the OR of samples for research projects with IRB approval and which relevance has been approved by the review committee of the Facility. This service has two components, the acquisition component in which the investigators request specific tissues that are collected and given to the researchers after the pathologist has established the diagnosis and the banking component, where we have stored tumor samples from several tissues of origin: brain, gynecological tumors, breast, prostate, bladder, and melanoma.
Histopathology and Immunohistochemistry: This resource provides paraffin embedding, sectioning, staining, cryostat sectioning, and automated immunohistochemistry of human and rodent tissue samples. The resource also provides a pathology consultation service.