The NYSBC is the nation’s premier center for structural biology with unrivaled facilities for Nuclear Magnetic Resonance Spectroscopy (NMR), Cryoelectron Microscopy (CEM), and X-Ray Crystallography. NYSBC’s research facilities include state-of-the-art instrumentation for analyzing proteins and other molecules that trigger diseases, such as bacterial and viral infections; disease and neurodegeneration; and trauma. The NYSBC is located at 133rd Street and Convent Avenue in Manhattan.
Public site: http://www.nysbc.org
Intranet site: http://www.nysbc.net
Core Facilities
11 High-field Nuclear Magnetic Resonance Spectrometers
4 Cryoelectron Microscopes
2 X-Ray Crystallographic “Beamlines” at NSLS
NYU Membership
NYU is a member of this consortium and users from all NYU departments can obtain access to this research instrumentation.
NMR Facilities
> 900 megahertz (MHz) four channel NMR system with cryoprobe
> 900 MHz US2 four channel NMR system with TXI and solid state probes
> 800 MHz US2 four channel NMR system with cryoprobe
> 800 MHz four channel NMR system with cryoprobe
> 800 MHz US2 four channel NMR system with cryoprobe
> 750 MHz 89 mm bore four channel solids NMR system with multiple CP-MAS and HRMAS probes
> 500 MHz four channel NMR system with cryoprobe
> 600 MHz four channel NMR system with cryoprobe
> 700 MHz four channel NMR system with cryoprobe
NYU Usage of NMR Facilities
NYU share is ~6.75 900MHz equivalent weeks/year.
It terms of calendar weeks one model usage pattern that equals 6.75 900MHz equivalent weeks would be to use ~ 1.35 weeks/instrument for the year as follows:
> 500 MHz - 1.35 weeks
> 600 MHz - 1.35 weeks
> 700 MHz - 1.35 weeks
> 750 MHz - 1.35 weeks
> 800#1 MHz - 1.35 weeks
> 800#2 MHz - 1.35 weeks
> 800conv MHz - 1.35 weeks
> 900#1 MHz - 1.35 weeks
> 900#2 MHz - 1.40 weeks
This model distributes the time (using the new weights) as evenly as possible over all instruments, but does not reflect actual usage. There are many usage patterns that would equal 6.75 900MHz equivalent weeks.
Another example that does not use fractional weeks looks like this:
> 500 MHz - 2.0 weeks
> 600 MHz - 1.0 weeks
> 700 MHz - 1.0 weeks
> 750 MHz - 2.0 weeks
> 800#1 MHz - 1.0 weeks
> 800#2 MHz - 2.0 weeks
> 800conv MHz - 2.0 weeks
> 900#1 MHz - 1.0 weeks
> 900#2 MHz - 1.0 weeks
Scheduling
The current instrument schedules can be looked up on the intranet site at http://www.nysbc.net/twiki/bin/view/Main/NmrSchedule (requires login). One can schedule for up to three months ahead, and a lead time of a month is typical. On occasion one can obtain slots via the waitlist (in case a user drops out). Instrument usage is allocated in units of weeks (Tuesday morning to Monday night). One may trade weeks or days between institutions. Note that reserved but unused slots will be charged as used to the usage account. The instruments can be used 24/7, although after hours and weekend/holiday physical access is limited to people with all-hours certification.
How to Sign-up
If you are interested in using the NMR facilities, please contact Dr. Chin Lin, chin.lin@nyu.edu, and/or Dr. Alexej Jerschow, alexej.jerschow@nyu.edu to discuss your needs. Frequent users may request to be trained at NYSBC for cryoprobe operations in which case they can gain operator status at NYSBC (for access M-F, 9am-5pm). All-hour access requires special certification.
Other Facilities (CryoEM and Beamline)
For CryoEM facilities, please contact Dr. David Stokes, stokes@saturn.med.nyu.edu. For beamline use, please contact Dr. Jon Schwanof schwanof@bnl.gov.
Acknowledging Use of NYSBC Facilities
Principal Investigators should acknowledge their use of NYSBC in papers and presentations as follows:
"Prof. X. Y. is a member of the New York Structural Biology Center. The Center is a STAR center supported by the New York State Office of Science, Technology, and Academic Research. "
> Staff will certainly appreciate your appropriate acknowledgment of their efforts. There is no general requirement for acknowledgment of staff performing their normal training, maintenance, and support activities.
> Some journals have restrictions on how individual grants may be acknowledged, and of course you may need to modify the statement for their restrictions.
NMR, General Facilities at 750 MHz and below
For NMR (for work done 2003- June 2008 ), add
"NMR resources supported by NIH P41 GM66354. "
900's
Add "900 MHz spectrometers were purchased with funds from NIH, USA, the Keck Foundation, New York State, and the NYC Economic Development Corporation."
CEM, JEOL 3200FSC microscope (300kV)
"300 kV electron microscope was purchased with funds from NIH grant S10 RR17291"