The Department's Core Imaging Facility has several different types of microscope systems that are housed in the Center for Genomics and System Biology and in the Brown Building. Currently, the Core Imaging Facility has two Leica SP5 point-scanning laser confocal microscope systems, and an Improvision Spinning Disk confocal microscope system. In addition to these confocal systems, there are several types of upright and inverted microscopes each configured with different types of optical and illumination systems. Image analysis is provided through dedicated computer workstations running the Leica LAS Analysis software, Volocity, and Metamorph software. The mixture of microscope systems located in the Imaging Facility provide microscope users a variety of ways to visualize and analyze their microscopic images.
The Department also has two specialized microscopes for microdissection and deconvolution imaging. A fluorescent capable Leica AS Laser Capture Dissection Microscope (LCM) allows cutting cells or sub-cellular structures as small as 5 microns. This system has been used to isolate cells for downstream RNA analysis. The LCM has also been used for laser microdissections on tissues as thick as 100 to 150 microns. The image analysis software Metamorph, is used to run an automated Nikon Elipse 901 fluorescent compound microscope. Equipped with high N.A. DIC and phase-contrast objectives, this system is fully automated to acquire a series of Z-stacks for time lapse imaging. The deconvolution capability of this microscope systems complements the confocal microscopes in the facility.
A number of dedicated confocal microscope systems are also found in various labs. These systems are overseen by the lab's principle investigator.
- Olympus FV1000 MPE Multi-Photon Microscope: This system is used by Dr. Claude Desplan's Lab for deep tissue imaging
- Leica SP8 Fast Tandem Confocal Scanner: This system is used to image life samples in Dr. Lionel Christiaen's Lab.
- Leica SPE Confocal Microscope: This system is used by Dr. Ken Birnbaum's Lab to image the process of root growth.
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Questions regarding these microscopes and reservations should be directed to Dr. Ignatius P. Tan (firstname.lastname@example.org).